User:Allison K. Alix/Notebook/Thesis Research/2013/08/29: Difference between revisions
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*One step of the procedure notes to centrifuge DNA and Sulfo-SMCC to remove excess SSMCC; however, after centrifugation, no separation between the two was observed. Because of this, it will be better to add the Sulfo-SMCC to the NH2 functionalized Au@SiO2 and then add the DNA last. | *One step of the procedure notes to centrifuge DNA and Sulfo-SMCC to remove excess SSMCC; however, after centrifugation, no separation between the two was observed. Because of this, it will be better to add the Sulfo-SMCC to the NH2 functionalized Au@SiO2 and then add the DNA last. | ||
* Procedure 1 has too many rinsing steps which lead me to believe many of the nanoparticles are lost. | |||
==New Procedure== | |||
1) Combine 1mL 5nM silica coated Au particles with 5uL 1mM APS. Stir for 1 hour. | |||
2) Add 2mg Sulfo-SMCC in 3mL KPS buffer (pH =7.4, 0.05M) add 1.5mL to NH2 functionalized AuNP's. | |||
3) Stir for 1hr. and centrifuge for ?? | |||
4) Add | |||
Revision as of 09:06, 29 August 2013
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Revisions to DNA attachment on Au@SiO2
New Procedure1) Combine 1mL 5nM silica coated Au particles with 5uL 1mM APS. Stir for 1 hour. 2) Add 2mg Sulfo-SMCC in 3mL KPS buffer (pH =7.4, 0.05M) add 1.5mL to NH2 functionalized AuNP's. 3) Stir for 1hr. and centrifuge for ?? 4) Add
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