User:Allison K. Alix/Notebook/Thesis Research/2013/08/29: Difference between revisions

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==Entry title==
==Revisions to DNA attachment on Au@SiO2==
* Insert content here...
 
*KP buffer was NOT at 7.4 (was at 7.13). pH was readjusted to 7.4 using ~2M NaOH.
 
*One step of the procedure notes to centrifuge DNA and Sulfo-SMCC to remove excess SSMCC; however, after centrifugation, no separation between the two was observed. Because of this, it will be better to add the Sulfo-SMCC to the NH2 functionalized Au@SiO2 and then add the DNA last.





Revision as of 07:38, 29 August 2013

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Revisions to DNA attachment on Au@SiO2

  • KP buffer was NOT at 7.4 (was at 7.13). pH was readjusted to 7.4 using ~2M NaOH.
  • One step of the procedure notes to centrifuge DNA and Sulfo-SMCC to remove excess SSMCC; however, after centrifugation, no separation between the two was observed. Because of this, it will be better to add the Sulfo-SMCC to the NH2 functionalized Au@SiO2 and then add the DNA last.