User:Allison K. Alix/Notebook/Thesis Research/2013/08/27

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(Au@SiO2/DNA UV-Vis Data)
(Procedures)
Line 27: Line 27:
c = 6.23x10^-7M = 0.624uM
c = 6.23x10^-7M = 0.624uM
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==Procedures==
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==Addition of ThT==
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1) Aliquot 150uL Au@SiO2/DNA solution into a clean epitest tube.
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[ThT] must be twice what [DNA] is
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2) Add 15uL x ThT to solution
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[DNA] = 1.11225μM, so [ThT] must be 2.2245μM
 +
Use 250μL of DNA-AuNP solution (10%=25μM)
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2.2245x10-6M x 275x10-6L solution = 6.117375x10-10 moles ThT
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3) Allow to stand for ~10 minutes
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(6.117375x10-10 moles ThT)/ 25 x 10-6 L = 24.4695μM
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-Add 25μL of 24.4695μM ThT to 250μL thiol-DNA/AuNP solution
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NEW Concentrations:
 +
 
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[DNA]= 1.011μM [AuNP] = 13.486μM [ThT] = 2.2245μM
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Revision as of 10:41, 27 August 2013

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To Do

  • Add Filter to FCS
  • Align using YG Fluorescent Beads
  • Add ThT to Au@SiO2/DNA solution
  • Obtain UV-Vis and Fluorescence of above solution

Au@SiO2/DNA UV-Vis Data

Image:DNA_method_2.jpg‎

ε of thiol-DNA= 607500 A=0.379 b=1 cm

A=εbc

c=A/εb

c = 6.23x10^-7M = 0.624uM

Addition of ThT

[ThT] must be twice what [DNA] is

[DNA] = 1.11225μM, so [ThT] must be 2.2245μM

Use 250μL of DNA-AuNP solution (10%=25μM)

2.2245x10-6M x 275x10-6L solution = 6.117375x10-10 moles ThT

(6.117375x10-10 moles ThT)/ 25 x 10-6 L = 24.4695μM

-Add 25μL of 24.4695μM ThT to 250μL thiol-DNA/AuNP solution

NEW Concentrations:

[DNA]= 1.011μM [AuNP] = 13.486μM [ThT] = 2.2245μM


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