User:Allison K. Alix/Notebook/Thesis Research/2013/08/27

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(Au@SiO2/DNA UV-Vis Data)
(Au@SiO2/DNA UV-Vis Data)
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c = 6.23x10^-7M = 0.624uM
c = 6.23x10^-7M = 0.624uM
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ThT must be at least double this concentration in solution, 1.25uM
 
==Procedures==
==Procedures==

Revision as of 10:41, 27 August 2013

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To Do

  • Add Filter to FCS
  • Align using YG Fluorescent Beads
  • Add ThT to Au@SiO2/DNA solution
  • Obtain UV-Vis and Fluorescence of above solution

Au@SiO2/DNA UV-Vis Data

Image:DNA_method_2.jpg‎

ε of thiol-DNA= 607500 A=0.379 b=1 cm

A=εbc

c=A/εb

c = 6.23x10^-7M = 0.624uM

Procedures

1) Aliquot 150uL Au@SiO2/DNA solution into a clean epitest tube.

2) Add 15uL x ThT to solution


3) Allow to stand for ~10 minutes


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