User:Allison K. Alix/Notebook/Thesis Research/2013/08/27: Difference between revisions
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ThT must be at least double this concentration in solution, 1.25uM | ThT must be at least double this concentration in solution, 1.25uM | ||
==Procedures== | |||
1) Aliquot 150uL Au@SiO2/DNA solution into a clean epitest tube. | |||
2) Add 15uL x ThT to solution | |||
3) Allow to stand for ~10 minutes | |||
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Revision as of 08:38, 27 August 2013
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To Do
Au@SiO2/DNA UV-Vis Dataε of thiol-DNA= 607500 A=0.379 b=1 cm A=εbc c=A/εb c = 6.23x10^-7M = 0.624uM ThT must be at least double this concentration in solution, 1.25uM Procedures1) Aliquot 150uL Au@SiO2/DNA solution into a clean epitest tube. 2) Add 15uL x ThT to solution
3) Allow to stand for ~10 minutes |