User:Allison K. Alix/Notebook/Thesis Research/2013/08/13
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Scaled up SilicaCoating ProcedureOriginal: 1mL AuNP & MHA solution/2μL TES/0.125mL NH4OH/5mL 2propanol 1) Rescale to use 9mL AuNP/MHA solution New: 9mL AuNP&MHA solution/18uL TES/1.125mL NH4OH/45mL 2propanol 2) Stir solutions for one hour to allow reaction to proceed 3) Centrifuge solutions at 4000 rpm for one hour 4) Resuspend particles in water CalculationsOriginal concentration of AuNP/MHA solution is 4.7nM 4.7nM(9mL)=55.143mL(x) x= 0.767nM 0.767x10^9moles/L x 1L/1000mL x 36mL = 2.7612 x 10^11 moles AuNP total 2.7612 x 10^11 moles AuNP x 6.02 x 10^23 particles/mole = 1.66 x 10^13 total Au nanoparticles
5x10^9 moles/L x 1L/1000mL x 6.02x10^23 particles/mole = 3.01 x 10^12 particles/mL 1.66 x 10^13 particles / x = 3.01 x10^12 particles/1 mL x = 5.52mL Dilute AuNP in 5.52mL water after removing supernantant 5.52mL/24 vials = 230uL per vial. DNA Attachment1) Mix 300uL AuNP@SiO2 with 30uL APS and 470uL 100% ethanol. Sonicate for 30 minutes
