# User:Allison K. Alix/Notebook/Thesis Research/2013/08/13

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==Scaled up Silica-Coating Procedure== | ==Scaled up Silica-Coating Procedure== | ||

- | Original: 1mL AuNP & MHA solution/2μL TES/0.125mL NH4OH/5mL propanol | + | Original: 1mL AuNP & MHA solution/2μL TES/0.125mL NH4OH/5mL 2-propanol |

1) Re-scale to use 9mL AuNP/MHA solution | 1) Re-scale to use 9mL AuNP/MHA solution | ||

- | New: 9mL AuNP&MHA solution/18uL TES/1.125mL NH4OH/45mL propanol | + | New: 9mL AuNP&MHA solution/18uL TES/1.125mL NH4OH/45mL 2-propanol |

2) Stir solutions for one hour to allow reaction to proceed | 2) Stir solutions for one hour to allow reaction to proceed | ||

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3) Centrifuge solutions at 4000 rpm for one hour | 3) Centrifuge solutions at 4000 rpm for one hour | ||

- | 4) Re-suspend particles in water | + | 4) Re-suspend particles in water |

==Calculations== | ==Calculations== |

## Revision as of 10:58, 13 August 2013

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## Scaled up Silica-Coating ProcedureOriginal: 1mL AuNP & MHA solution/2μL TES/0.125mL NH4OH/5mL 2-propanol 1) Re-scale to use 9mL AuNP/MHA solution New: 9mL AuNP&MHA solution/18uL TES/1.125mL NH4OH/45mL 2-propanol 2) Stir solutions for one hour to allow reaction to proceed 3) Centrifuge solutions at 4000 rpm for one hour 4) Re-suspend particles in water ## CalculationsOriginal concentration of AuNP/MHA solution is 4.7nM 4.7nM(9mL)=55.143mL(x) x= 0.767nM 0.767x10^-9moles/L x 1L/1000mL x 55.143mL = 4.23 x 10^-11 moles AuNP total 4.23 x 10^-11 moles AuNP x 6.02 x 10^23 particles/mole = - We want a 5nM solution
5x10^-9 moles/L x 1L/1000mL x 6.02x10^23 particles/mole = 3.01 x 10^12 particles/mL 2.55 x 10^13 particles / x = 3.01 x10^12 particles/1 mL x = 8.47mL Dilute AuNP in 8.47mL water after removing supernantant 8.47mL/24 vials = 353uL per vial. ## DNA Attachment |