User:Allison K. Alix/Notebook/Thesis Research/2013/07/25: Difference between revisions
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==ThT/DNA attachment== | ==ThT/DNA attachment== | ||
1) Prepare | 1) Prepare 100uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively) | ||
a) 100nM | a) 100nM | ||
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d) 700nM | d) 700nM | ||
e) | e) 900nM | ||
***Original Concentrations of 4 and 8-repeats are 265.6uM and 142.8uM, respectively*** | |||
4-repeat | |||
1uM(1mL)=(265.6uM)x | |||
x = 3.77uL in 996.23uL | |||
900nM--> 90uL 1uM in 10uL PBS | |||
700nM--> 70uL in 30uL | |||
500nM--> 50uL in 50uL | |||
300nM--> 30uL in 70uL | |||
100nM--> 10uL in 90uL | |||
Revision as of 08:09, 25 July 2013
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To Do
Silica-Coating Part 11) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature prior to dilution*) 2) ThT/DNA attachment1) Prepare 100uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively) a) 100nM b) 300nM c) 500nM d) 700nM e) 900nM
4-repeat 1uM(1mL)=(265.6uM)x x = 3.77uL in 996.23uL 900nM--> 90uL 1uM in 10uL PBS 700nM--> 70uL in 30uL 500nM--> 50uL in 50uL 300nM--> 30uL in 70uL 100nM--> 10uL in 90uL
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