User:Allison K. Alix/Notebook/Thesis Research/2013/07/25: Difference between revisions
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[100-([x(100)]/5.31)] = | [100-([x(100)]/5.31)] = | ||
1800nM--> 33.9uL in | 1800nM--> 33.9uL in 66.1uL PBS | ||
1400nM--> 26.4uL in | 1400nM--> 26.4uL in 73.6uL | ||
1000nM--> 18.8uL in | 1000nM--> 18.8uL in 81.2uL | ||
600nM--> 11.3uL in | 600nM--> 11.3uL in 88.7uL | ||
200nM--> 3.8uL in | 200nM--> 3.8uL in 96.2uL | ||
'''8-repeat''' | '''8-repeat''' | ||
Revision as of 09:31, 25 July 2013
 Project name
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To Do
Silica-Coating Part 11) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature prior to dilution*) 2) ThT/DNA attachment1) Prepare 100uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively) a) 100nM b) 300nM c) 500nM d) 700nM e) 900nM NOTE: Original Concentrations of 4 and 8-repeats are 265.6uM and 142.8uM, respectively 4-repeat x(500uL)=(265.6uM)(10) x = 5.31uM [100-([x(100)]/5.31)] = 1800nM--> 33.9uL in 66.1uL PBS 1400nM--> 26.4uL in 73.6uL 1000nM--> 18.8uL in 81.2uL 600nM--> 11.3uL in 88.7uL 200nM--> 3.8uL in 96.2uL 8-repeat 142.8uM(10uL) = (x)(500uL) x= 2.856uM 1800nM--> 63uL in 37uL PBS 1400nM--> 49uL in 51uL 1000nM--> 35uL in 65uL 600nM--> 21uL in 79uL 200nM--> 7uL in 93uL
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