User:Allison K. Alix/Notebook/Thesis Research/2013/07/25

From OpenWetWare
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

To Do

  • Repeat Silica-Coating procedure using water to dilute nanoparticles instead of PBS. PBS is likely the cause of the aggregation we have been observing.[1]
  • Repeat ThT-DNA fluorescence procedure to show that fluorescence increases with increasing number of G-quadruplexes formed.

Silica-Coating Part 1

1) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature prior to dilution*)

2)

ThT/DNA attachment

1) Prepare 100uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively)

a) 100nM

b) 300nM

c) 500nM

d) 700nM

e) 900nM

NOTE: Original Concentrations of 4 and 8-repeats are 265.6uM and 142.8uM, respectively

4-repeat

x(500uL)=(265.6uM)(8.12)

x = 4.31uM

1800nM--> 41.8uL in 58.2uL PBS

1400nM--> 32.5uL in 67.5uL

1000nM--> 23.2uL in 76.8uL

600nM--> 13.9uL in 86.1uL

200nM--> 4.6uL in 95.4uL

8-repeat

142.8uM(10uL) = (x)(500uL)

x= 2.856uM

1800nM--> 63uL in 37uL PBS

1400nM--> 49uL in 51uL

1000nM--> 35uL in 65uL

600nM--> 21uL in 79uL

200nM--> 7uL in 93uL






Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Allison_K._Alix/Notebook/Thesis_Research/2013/07/25&oldid=712149"