User:Allison K. Alix/Notebook/Thesis Research/2013/07/15: Difference between revisions

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==Silica Coating Procedure: Part 1==
==Silica Coating Procedure: Part 1==


* Prior to addition of TES to AuNP, MHA must be attached to AuNP
* We have decided to use the procedure adapted from [http://pubs.acs.org/doi/pdf/10.1021/ac0600555] to coat the AuNP with Silica. The first step is to attach a ligand stabilizer, mercaptohexadecanoic acid, MHA, to the nanoparticles, as described below.


1) Dissolve 0.0144g MHA in 5mL PBS, combine with 5mL AuNPs
1) Dissolve 0.0144g MHA in 5mL PBS (10mM MHA), combine with 5mL AuNPs


2) Stir overnight
2) Stir overnight




Revision as of 07:53, 15 July 2013

DNA/AuNP attachment & Silica Coating Pt 1 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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DNA/AuNP Attachment

1) Prepare 2 solutions containing:

  • 250uL 4.45uM thiol DNA + 250uL 100mM DTT in 4% TEA

2) React 10 minutes and extract DNA using ethyl acetate

3) Prepare the following two solutions

A) 240.7uL 29.67nM AuNP + 240.7uL 2.225uM DNA + 18.7uL sodium citrate

  • Final concentrations: [AuNP]=14.28nM, [DNA]=1.071uM, [sodium citrate]=10mM

B) 157.5uL 29.67nM auNP + 157.5uL 2.225uM DNA + 35uL sodium citrate

  • Final Concentrations: [AuNP]= 13.35nM, [DNA]=1.0014uM, [sodium citrate]=10mM

4) React 10 minutes

5) Centrifuge for 4 minutes at 7000rpm, redisperse in 50mM HEPES buffer

Silica Coating Procedure: Part 1

  • We have decided to use the procedure adapted from [1] to coat the AuNP with Silica. The first step is to attach a ligand stabilizer, mercaptohexadecanoic acid, MHA, to the nanoparticles, as described below.

1) Dissolve 0.0144g MHA in 5mL PBS (10mM MHA), combine with 5mL AuNPs

2) Stir overnight








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