User:Allison K. Alix/Notebook/Thesis Research/2013/07/15: Difference between revisions

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2) React 10 minutes and extract DNA using ethyl acetate
2) React 10 minutes and extract DNA using ethyl acetate


3) Prepare the following two solutions
3) Prepare the following two solutions  
 
A) 240.7uL 29.67nM AuNP + 240.7uL 2.225uM DNA + 18.7uL sodium citrate
 
* Final concentrations: [AuNP]=14.28nM, [DNA]=1.071uM, [sodium citrate]=10mM
 
 
 


A)




Revision as of 07:32, 15 July 2013

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DNA/AuNP Attachment

1) Prepare 2 solutions containing:

  • 250uL 4.45uM thiol DNA + 250uL 100mM DTT in 4% TEA

2) React 10 minutes and extract DNA using ethyl acetate

3) Prepare the following two solutions

A) 240.7uL 29.67nM AuNP + 240.7uL 2.225uM DNA + 18.7uL sodium citrate

  • Final concentrations: [AuNP]=14.28nM, [DNA]=1.071uM, [sodium citrate]=10mM







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