User:Allison K. Alix/Notebook/Thesis Research/2013/07/11: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> DNA/AuNP attachment</span> | ||
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Revision as of 11:34, 12 July 2013
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To Do
Calculating DNA and AuNP concentrations
x = 1.455uM <-----this is the concentration prior to mixing with AuNP/sodium citrate 1.455uM(500uL) = x (250uL) x = 2.91uM <---this is the concentration prior to mixing with 250uL 100mM DTT in 4% TEA Concentration of stock DNA = 5.31uM 5.31uM(x) = 2.91uM(250uL) x = 137uL in 113uL PBS Preparation of 1X PBSMake 500mL 1X from 10X stock solution 1X(500mL)=(10X)(x) x = 50mL in 450mL Preparation of 284mM sodium citrate buffer[Base] = sodium citrate dihydrate, FW= 294.1 g/mol [Acid] = citric acid, FW= 210.14 g/mol pH = pKa + log [base]/[acid] 3.0 = 3.14 + log [base]/[acid] -0.14 = log [base]/[acid] [base]/[acid] = 0.7244 [acid] + [base] = 284mM [base] = 284mM - [acid] 284mM-[acid]/[acid] = 0.7244 [acid]= 0.165M [base]= 0.119M 0.165mol/L x 0.5L x 210.14g/mol = 17.30g citric acid 0.1193mol/L x 0.5L x 294.1g/mol = 17.54g sodium citrate dihydrate 1) Add 17.3g citric acid and 17.54g sodium citrate dihydrate to ~400mL of water 2) Adjust pH to 3.0 using 1M HCl 3) Adjust volume to 500mL using milliQ water
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