User:Allison K. Alix/Notebook/Thesis Research/2013/07/10: Difference between revisions

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3) Extract DNA using 4 2mL aliquots of ethyl acetate
3) Extract DNA using 4 2mL aliquots of ethyl acetate
4) Add 232.4uL 29.67nM AuNP to 232.4uL 2.22uM DNA
5) React 5 min
6) Add 35.2uL sodium citrate buffer
7) React 10 minutes
8) Centrifuge 3 times for 10000rpm for 3 minutes. Redisperse all supernatants in 50mM HEPES buffer.
9) Take UV-Vis of AuNP/DNA sample and all supernatants.




Revision as of 08:50, 10 July 2013

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DNA-AuNP attachment

  • This will be used to calculate the amount of DNA that is adsorbing onto the gold nanoparticles. I will be able to compare this amount to the amount being adsorbed onto the silica coated gold nanoparticles to get an idea of whether or not the silica hinders that attachment and if so by how much. Will I need to add more DNA?

Follow procedure from User:Allison_K._Alix/Notebook/Thesis_Research/2013/04/15

Procedures:

1) Mix 250uL 4.405uM DNA with 250uL 100mM DTT in 4% TEA

2) React for 10 minutes

3) Extract DNA using 4 2mL aliquots of ethyl acetate

4) Add 232.4uL 29.67nM AuNP to 232.4uL 2.22uM DNA

5) React 5 min

6) Add 35.2uL sodium citrate buffer

7) React 10 minutes

8) Centrifuge 3 times for 10000rpm for 3 minutes. Redisperse all supernatants in 50mM HEPES buffer.

9) Take UV-Vis of AuNP/DNA sample and all supernatants.




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