User:Allison K. Alix/Notebook/Thesis Research/2013/07/02: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> FCS Measurements</span> | ||
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== | ==FCS Measurements== | ||
* | |||
Prepare the following oligio-D samples: | |||
1) 150pM | |||
2) 125pM | |||
3) 100pM | |||
Prepare the following DNA/MB samples | |||
1) 100pM/80pM | |||
2) 75pM/60pM | |||
3) 50pM/40pM | |||
*Each sample was run 3 times for 500 seconds | |||
==FCS Data== | |||
[[Image:Green Beads image OWW.png]] | |||
The above image show an average correlation curve of 10,000x diluted green fluorescent beads | |||
[[Image:100pM OD OWW.png]] | |||
[[Image:125pM OD OWW.png]] | |||
[[Image:150pM OD OWW.png]] | |||
[[Image:50pM DNA OWW.png]] | |||
[[Image:75pM DNA OWW.png]] | |||
[[Image:100pM DNA OWW.png]] | |||
==Calibration Curves== | |||
[[Image:OD Cali Curve OWW.png]] | |||
The above image is a calibration curve of 1/N vs. concentration for the Oligo-D samples. We observed a very linear relationship between these samples, with an R<sup>2</sup> value of 0.99. | |||
[[Image:DNA MB Cali Curve OWW.png]] | |||
The above image is a calibration curve of 1/N vs. concentration for the DNA/MB samples. The 100pM and 75pM samples seem to follow the linear trend in relation to one another, however, the 50pM sample is a clear outlier. Because we did not see the pattern we are expecting for all three samples, we will prepare fresh solution tomorrow and run them again to see if there was some kind of error in sample preparation. | |||
Revision as of 11:36, 12 July 2013
 FCS Measurements
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FCS MeasurementsPrepare the following oligio-D samples: 1) 150pM 2) 125pM 3) 100pM Prepare the following DNA/MB samples 1) 100pM/80pM 2) 75pM/60pM 3) 50pM/40pM
FCS Data
The above image show an average correlation curve of 10,000x diluted green fluorescent beads
Calibration Curves
The above image is a calibration curve of 1/N vs. concentration for the Oligo-D samples. We observed a very linear relationship between these samples, with an R2 value of 0.99.
The above image is a calibration curve of 1/N vs. concentration for the DNA/MB samples. The 100pM and 75pM samples seem to follow the linear trend in relation to one another, however, the 50pM sample is a clear outlier. Because we did not see the pattern we are expecting for all three samples, we will prepare fresh solution tomorrow and run them again to see if there was some kind of error in sample preparation.
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