User:Allison K. Alix/Notebook/Thesis Research/2013/06/28

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FCS Measurements

  • Run the following samples of MB/DNA

1) 10nM MB + 8nM DNA

2) 5nM MB 4nM DNA

3) 1nM MB 960pM DNA

4) 500pM MB 400pM DNA

5) 100pM MB 80pM DNA

6) 50pM MB 40pM MB

7) 25pM MB 20pM DNA

  • Run the following oligio-D samples

1) 20pM

2) 40pM

3) 60pM

4) 80pM

5) 100pM

Calculations

MOLECULAR BEACON:

  • 7.61μM (x) = 20nM (1000uL)

x = 2.62uL 7.61uM MB in 997.38uL PBS

  • 20nM (x) = (30uL)(10nM)

15uL in 15uL PBS

  • 20nM (x) = 2nM(100uL)

10uL in 90uL

  • 2nM (x) = 1nM (100uL)

x = 50uL in 50uL PBS

  • 200pM (100uL) = x (1nM)

x = 20uL in 80uL PBS

  • 100pM(100uL) = (200pM)(x)

x = 50uL in 50uL buffer

  • 50pM (30uL) = 100pM (x)

x = 15uL in 15uL

DNA:

  • 5.31uM (500uL) = x (53.1uM)

x = 50uL in 450uL PBS

  • 5.31uM (x) = 16nM (1000uL)

x = 3uL in 997uL PBS

  • 16nM (x) = (8nM) (100uL)

x = 50uL in 50uL PBS

  • 1.92nM (100uL) = x (8nM)

x = 24uL in 76 uL PBS

  • 0.8nM (100uL) = x (1.92nM)

x = 41.7uL in 58.3uL

  • 160pM (100) = x (800pM)

x = 20uL in 80uL

  • 80pM (100uL) = x (160pM)

x = 50uL in 50uL

  • 40pM (30uL) = x (80pm)

x = 15uL in 15uL PBS

OLIGIO-D

  • 10uM (x) = 1500uL (20000pM)

x = 3uL in 1497uL PBS

  • 20000pM (x) = 1000pM (100uL)

5 uL in 95 uL PBS

  • 1000pM (x) = 260uL (100pM)

26uL in 234uL PBS

  • 80pM = 80uL 100pM in 20uL PBS
  • 60pM = 60uL 100pM in 40uL PBS
  • 40pM = 40uL 100pM in 60uL PBS
  • 20pM = 20uL 100pM in 80uL PBS





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