User:Allison K. Alix/Notebook/Thesis Research/2013/06/26: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 14: Line 14:
==13.7nm AuNP synthesis==
==13.7nm AuNP synthesis==


* The nanoparticles produced from this reaction will be used in the silica coating procedure in the future. We are interested in finding a procedure for synthesizing AuNP that has a low diameter variation. The current procedure has a wide range (5-40nm) that greatly affects the amount of PVP/TES that need to be added for the silica coating procedure. Minimizing changes in variables will help simplify the procedure.
1) Add 1mL 0.25mM HAuCl<sub>4</sub> to 100mL water


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 18:51, 26 June 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Preparation of DNA/MB Samples for FCS

Prepare the following samples:

1) 100pM DNA

13.7nm AuNP synthesis

  • The nanoparticles produced from this reaction will be used in the silica coating procedure in the future. We are interested in finding a procedure for synthesizing AuNP that has a low diameter variation. The current procedure has a wide range (5-40nm) that greatly affects the amount of PVP/TES that need to be added for the silica coating procedure. Minimizing changes in variables will help simplify the procedure.

1) Add 1mL 0.25mM HAuCl4 to 100mL water