User:Allison K. Alix/Notebook/Thesis Research/2013/06/26

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(Preparation of DNA/MB Samples for FCS)
Current revision (14:37, 12 July 2013) (view source)
 
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{|{{table}} width="800"
{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> DNA/MB and AuNP Prep</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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Prepare the following samples:
Prepare the following samples:
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1) 100pM DNA
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1) 50nM MB + 40nM DNA
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2) 10nM MB + 8nM DNA
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3) 5nM MB + 4nM DNA
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4) 500pM MB + 400pM DNA
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5) 100pM MB + 80pM DNA
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6) 50pM MB + 40pM DNA
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7) 20pM MB + 16pM DNA
==13.7nm AuNP synthesis==
==13.7nm AuNP synthesis==
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* The nanoparticles produced from this reaction will be used in the silica coating procedure in the future. We are interested in finding a procedure for synthesizing AuNP that has a low diameter variation. The current procedure has a wide range (5-40nm) that greatly affects the amount of PVP/TES that need to be added for the silica coating procedure. Minimizing changes in variables will help simplify the procedure.
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1) Add 1mL 0.25mM HAuCl<sub>4</sub> to 100mL water. Stir and heat to boiling.
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Current [HAuCl<sub>4</sub>] = 1mM
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1mM(x) = (0.25mM)(1mL)
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x = 250μL 1mM HAuCl<sub>4</sub> in 750μL H<sub>2</sub>O
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2) Add 4.0mL 1% sodium citrate (freshly prepared??)
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1g sodium citrate in 100mL of H<sub>2</sub>O
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3) Observe a color change to "red wine"
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Current revision

DNA/MB and AuNP Prep Main project page
Previous entry      Next entry

Preparation of DNA/MB Samples for FCS

Prepare the following samples:

1) 50nM MB + 40nM DNA

2) 10nM MB + 8nM DNA

3) 5nM MB + 4nM DNA

4) 500pM MB + 400pM DNA

5) 100pM MB + 80pM DNA

6) 50pM MB + 40pM DNA

7) 20pM MB + 16pM DNA

13.7nm AuNP synthesis

  • The nanoparticles produced from this reaction will be used in the silica coating procedure in the future. We are interested in finding a procedure for synthesizing AuNP that has a low diameter variation. The current procedure has a wide range (5-40nm) that greatly affects the amount of PVP/TES that need to be added for the silica coating procedure. Minimizing changes in variables will help simplify the procedure.

1) Add 1mL 0.25mM HAuCl4 to 100mL water. Stir and heat to boiling.

Current [HAuCl4] = 1mM

1mM(x) = (0.25mM)(1mL)

x = 250μL 1mM HAuCl4 in 750μL H2O

2) Add 4.0mL 1% sodium citrate (freshly prepared??)

1g sodium citrate in 100mL of H2O

3) Observe a color change to "red wine"




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