User:Allison K. Alix/Notebook/Thesis Research/2013/06/26: Difference between revisions
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* The nanoparticles produced from this reaction will be used in the silica coating procedure in the future. We are interested in finding a procedure for synthesizing AuNP that has a low diameter variation. The current procedure has a wide range (5-40nm) that greatly affects the amount of PVP/TES that need to be added for the silica coating procedure. Minimizing changes in variables will help simplify the procedure. | * The nanoparticles produced from this reaction will be used in the silica coating procedure in the future. We are interested in finding a procedure for synthesizing AuNP that has a low diameter variation. The current procedure has a wide range (5-40nm) that greatly affects the amount of PVP/TES that need to be added for the silica coating procedure. Minimizing changes in variables will help simplify the procedure. | ||
1) Add 1mL 0.25mM HAuCl<sub>4</sub> to 100mL water | 1) Add 1mL 0.25mM HAuCl<sub>4</sub> to 100mL water. Stir and heat to boiling. | ||
Current [HAuCl<sub>4</sub>] = 1mM | |||
1mM(x) = (0.25mM)(1mL) | |||
x = 250μL 1mM HAuCl<sub>4</sub> in 750μL H<sub>2</sub>O | |||
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Revision as of 18:55, 26 June 2013
 Project name
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Preparation of DNA/MB Samples for FCSPrepare the following samples: 1) 100pM DNA 13.7nm AuNP synthesis
1) Add 1mL 0.25mM HAuCl4 to 100mL water. Stir and heat to boiling. Current [HAuCl4] = 1mM 1mM(x) = (0.25mM)(1mL) x = 250μL 1mM HAuCl4 in 750μL H2O
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