User:Allison K. Alix/Notebook/Thesis Research/2013/06/05: Difference between revisions
(→Data) |
|||
Line 20: | Line 20: | ||
NOTE: TES solution will be prepared immediately prior to use to minimize exposure | NOTE: TES solution will be prepared immediately prior to use to minimize exposure | ||
'''Part 2: Preparing ZnPPIX solutions''' | '''Part 2: Preparing ZnPPIX solutions''' | ||
Line 26: | Line 27: | ||
2.22μM ZnPPIX and 1.11265μM DNA | ''2.22μM ZnPPIX and 1.11265μM DNA'' | ||
Line 38: | Line 39: | ||
ZnPPIX, DNA and AuNP | ''ZnPPIX, DNA and AuNP'' | ||
Line 51: | Line 52: | ||
(5.31476×10-10moles)/(25×10-6L)=21.3μM ZnPPIX | (5.31476×10-10moles)/(25×10-6L)=21.3μM ZnPPIX | ||
* Make 21.3μM ZnPPIX: | |||
Make 21.3μM ZnPPIX: | |||
(5000μM)V=(21.3μM)(25μL) | (5000μM)V=(21.3μM)(25μL) | ||
Line 58: | Line 58: | ||
V=0.107μL in 24.9μL | V=0.107μL in 24.9μL | ||
* Add 25μL of 21.3μM ZnPPIX to 250μL DNA/AuNP solution | |||
Add 25μL of 21.3μM ZnPPIX to 250μL DNA/AuNP solution | |||
Line 71: | Line 70: | ||
2.22μM ZnPPIX | ''2.22μM ZnPPIX'' | ||
(5μM)V=(2.22μM)(300μL) | (5μM)V=(2.22μM)(300μL) |
Revision as of 09:10, 5 June 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objectives
ProceduresPart 1: Preparing solutions for coating AuNP with Silica 1) dissolve 0.716g PVP (10kg/mol) in 10mL water (0.00716M) 2) Dissolve 0.42mL NH4OH (29.3wt% NH3 in H2O) in 9.58mL Ethanol (200 proof) (4.2vol%) NOTE: TES solution will be prepared immediately prior to use to minimize exposure
Zinc protoporphyrin (ZnPPIX) samples will be prepared at the same concentrations, following the same procedure as the ThT samples.
V=266.4μL 5μM ZnPPIX in 33.6μL (53.1μ)V=(2.2253μM)(300μL) V=12.57μL DNA in 287.43μL
10% of 250μL=25μL (within 10% allows for accurate concentration comparison) Total solution volume: 275μL (2.068×10-6M)(275×10-6L)=5.31476×10-10moles ZnPPIX (5.31476×10-10moles)/(25×10-6L)=21.3μM ZnPPIX
(5000μM)V=(21.3μM)(25μL) V=0.107μL in 24.9μL
DNA- 0.94μM AuNP- 12.55nM ZnPPIX- 1.9364μM
V=133.2μL ZnPPIX in 166.8μL Data
|