User:Allison K. Alix/Notebook/Thesis Research/2013/05/23
Observe Fluor w/ and w/o AuNP | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objectives
ProceduresPart 1: Adding ThT to thiol-DNA/AuNP [ThT] must be twice what [DNA] is [DNA] = 1.11225μM, so [ThT] must be 2.2245μM Use 250μL of DNA-AuNP solution (10%=25μM) 2.2245x10-6M x 275x10-6L solution = 6.117375x10-10 moles ThT (6.117375x10-10 moles ThT)/ 25 x 10-6 L = 24.4695μM -Add 25μL of 24.4695μM ThT to 250μL thiol-DNA/AuNP solution NEW Concentrations: [DNA]= 1.011μM [AuNP] = 13.486μM [ThT] = 2.2245μM Take Fluorescence Spectrum Part 2: Preparing thiol-DNA/ThT solution using same concentrations in thiol-DNA/AuNP/ThT solution 1) Prepare 250μL of 2.022μM thiol DNA (5.82μM)(x) = (250μL)(2.022μM) x = 86.85 μL 5.82μM thiol-DNA in 163.15μL PBS Buffer 2) Prepare 250μL of 4.449μM ThT (5μM)(x) = (4.449μM)(250μL) x = 222.45μL in 27.55μL PBS Buffer 3) Mix 250μL 2.002μM thiol-DNA with 250μL 4.449μM ThT 4) Obtain Fluorescence Spectrum Data
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