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Project name
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Objectives
- React thiol-DNA/ThT/AuNP at 150:1 concentrations of thiol-DNA:AuNP
- Run 2% agarose gel on thiol-DNA/ThT/AuNP synthesized on 04/23/2013
- Take absorbance and fluorescence measurements of thiol-DNA/ThT before and after attaching AuNP
Procedures
- New AuNP synthesized is 29.67nM with a diameter of 10nm
Previous concentrations used:
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Initial |
Final
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thiol-DNA |
5.82μM |
727.5nM
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ThT |
5μM |
625nM
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AuNP |
19.4nM |
9.7nM
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To be used:
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Initial |
Final
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thiol-DNA |
17.8μM |
2.225μM
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ThT |
20μM |
2.5μM
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AuNP |
29.67nM |
14.835nM
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