User:Allison K. Alix/Notebook/Thesis Research/2013/05/14

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Procedures)
(Procedures)
Line 21: Line 21:
{| class="wikitable"
{| class="wikitable"
|-
|-
-
! Sample !! Initial !! Final
+
! !! Initial !! Final
|-
|-
| thiol-DNA || 5.82μM || 727.5nM
| thiol-DNA || 5.82μM || 727.5nM
Line 33: Line 33:
{| class="wikitable"
{| class="wikitable"
|-
|-
-
! Sample !! Initial !! Final
+
! !! Initial !! Final
|-
|-
| thiol-DNA || 17.8μM || 2.225μM
| thiol-DNA || 17.8μM || 2.225μM

Revision as of 09:46, 14 May 2013

Project name Main project page
Previous entry      Next entry

Objectives

  • React thiol-DNA/ThT/AuNP at 150:1 concentrations of thiol-DNA:AuNP
  • Run 2% agarose gel on thiol-DNA/ThT/AuNP synthesized on 04/23/2013
  • Take absorbance and fluorescence measurements of thiol-DNA/ThT before and after attaching AuNP

Procedures

  • New AuNP synthesized is 29.67nM with a diameter of 10nm

Previous concentrations used:

Initial Final
thiol-DNA 5.82μM 727.5nM
ThT 5μM 625nM
AuNP 19.4nM 9.7nM

To be used:

Initial Final
thiol-DNA 17.8μM 2.225μM
ThT 20μM 2.5μM
AuNP 29.67nM 14.835nM



Personal tools