User:Allison K. Alix/Notebook/Thesis Research/2013/05/14

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* New AuNP synthesized is 29.67nM with a diameter of 10nm
* New AuNP synthesized is 29.67nM with a diameter of 10nm
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Previous Concentrations Used
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Previous concentrations used:
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Revision as of 09:46, 14 May 2013

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Objectives

  • React thiol-DNA/ThT/AuNP at 150:1 concentrations of thiol-DNA:AuNP
  • Run 2% agarose gel on thiol-DNA/ThT/AuNP synthesized on 04/23/2013
  • Take absorbance and fluorescence measurements of thiol-DNA/ThT before and after attaching AuNP

Procedures

  • New AuNP synthesized is 29.67nM with a diameter of 10nm

Previous concentrations used:

Sample Initial Final
thiol-DNA 5.82μM 727.5nM
ThT 5μM 625nM
AuNP 19.4nM 9.7nM

To be used:

Sample Initial Final
thiol-DNA 17.8μM 2.225μM
ThT 20μM 2.5μM
AuNP 29.67nM 14.835nM



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