User:Allison K. Alix/Notebook/Thesis Research/2013/05/14

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(Procedures)
(Procedures)
Line 28: Line 28:
|-
|-
| AuNP || 19.4nM || 9.7nM
| AuNP || 19.4nM || 9.7nM
 +
|}
 +
 +
To be used:
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{| class="wikitable"
 +
|-
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! Sample !! Initial !! Final
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|-
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| thiol-DNA || 17.8μM || 2.225μM
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|-
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| ThT || 20μM || 2.5μM
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|-
 +
| AuNP || 29.67nM || 14.835nM
|}
|}

Revision as of 10:46, 14 May 2013

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Objectives

  • React thiol-DNA/ThT/AuNP at 150:1 concentrations of thiol-DNA:AuNP
  • Run 2% agarose gel on thiol-DNA/ThT/AuNP synthesized on 04/23/2013
  • Take absorbance and fluorescence measurements of thiol-DNA/ThT before and after attaching AuNP

Procedures

  • New AuNP synthesized is 29.67nM with a diameter of 10nm

Previous Concentrations Used

Sample Initial Final
thiol-DNA 5.82μM 727.5nM
ThT 5μM 625nM
AuNP 19.4nM 9.7nM

To be used:

Sample Initial Final
thiol-DNA 17.8μM 2.225μM
ThT 20μM 2.5μM
AuNP 29.67nM 14.835nM



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