User:Allison K. Alix/Notebook/Thesis Research/2013/04/23: Difference between revisions
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==Objectives== | ==Objectives== | ||
*Run 2% Gel with DNA-AuNP samples and supernatants | |||
*Measure abs/fluor of thiol DNA/ThT | |||
*Attach AuNP to thiol DNA/ThT | |||
==Procedures== | ==Procedures== |
Revision as of 09:08, 23 April 2013
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Objectives
ProceduresPart 1: Run 2% agarose gel with the following samples: 1)5uL 100bp ladder + 1uL loading buffer 2)5uL DNA-AuNP + 1uL loading buffer 3)5uL sup 1 + 1uL loading buffer 4)5uL sup 2 + 1uL loading buffer 5)5uL sup 3 + 1uL loading buffer 6)5uL sup 4 + 1uL loading buffer Part 2: Take Absorbance and Fluorescence of thiol DNA-ThT sample Part 3: React thiol-DNA/ThT with AuNP
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