User:Allison K. Alix/Notebook/Thesis Research/2013/04/23

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(Procedures)
Current revision (14:57, 13 May 2013) (view source)
(Procedures)
 
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Use procedure from [[User:Allison K. Alix/Notebook/Thesis Research/2013/04/15|04/15/2013]]
Use procedure from [[User:Allison K. Alix/Notebook/Thesis Research/2013/04/15|04/15/2013]]
-
original concentration of DNA in ThT: 2.91uL and 2.5uM
+
original concentration of DNA in ThT: 2.91uM and 2.5uM
after mixing with TEA: 1.45uM and 1.25uM
after mixing with TEA: 1.45uM and 1.25uM
after mixing with aunP: 727.5nM and 0.625uM ThT with 9.7nM AuNP
after mixing with aunP: 727.5nM and 0.625uM ThT with 9.7nM AuNP

Current revision

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Objectives

  • Run 2% Gel with DNA-AuNP samples and supernatants
  • Measure abs/fluor of thiol DNA/ThT
  • Attach AuNP to thiol DNA/ThT

Procedures

Part 1:

Run 2% agarose gel with the following samples:

1)5uL 100bp ladder + 1uL loading buffer

2)5uL DNA-AuNP + 1uL loading buffer

3)5uL sup 1 + 1uL loading buffer

4)5uL sup 2 + 1uL loading buffer

5)5uL sup 3 + 1uL loading buffer

6)5uL sup 4 + 1uL loading buffer

Part 2: Take Absorbance and Fluorescence of thiol DNA-ThT sample

Part 3: React thiol-DNA/ThT with AuNP

Use procedure from 04/15/2013

original concentration of DNA in ThT: 2.91uM and 2.5uM after mixing with TEA: 1.45uM and 1.25uM after mixing with aunP: 727.5nM and 0.625uM ThT with 9.7nM AuNP

Data

Image:Absorbance.png

Image:Fluor.png



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