User:Allison K. Alix/Notebook/Thesis Research/2013/04/16: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Gel Electrophoresis/Abs/thiol-DNA-ThT Rxn</span> | ||
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Revision as of 09:37, 16 April 2013
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Objectives
ProceduresPart one: Reconstituting DNA samples in water 1) Remove epi-test tubes from lyophilizer 2) Add 10 μL water to each sample and vortex to dissolve Part 2: Gel Electrophoresis. 1) Follow procedure outlined on 03/29/2013 2) Prepare the following 7 samples:
3) Load into gel 4) Stain with ehtidium bromide 5) Observe under UV Light Part 3: Preparing thiol-DNA/ThT solution
1) Dilute stock DNA (53.1μM) to 5.82μM 53.1μM DNA (x) = (500μL) (5.82μM) X = 54.8micL in 445.2 mic buffer 2) Mix 250μL thiol-DNA with 250μL 5μMThT 3) Heat at ~75°C for 25 minutes 4) Cool to room temperature
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