User:Allison K. Alix/Notebook/Thesis Research/2013/04/16: Difference between revisions
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==Observations== | |||
The absorbance data did not show a trend. The thiol-DNA/ThT/AuNP sample itself was the faintest shade of purple, which may be one reason we did not observe a distinguishable peak. Because of this, we decided to re-do the thiol-DNA/ThT reaction using a higher concentration of DNA (See Part 3 of the procedures). This allowed us to work with a higher concentration of gold nanoparticles since it's concentration is based off of the concentration of DNA used. | |||
==Notes== | |||
* We are still having trouble observing the gel under UV light. It is difficult to even see streaks due to the ladders. Because of this, we are going to focus on making a gel using different volumes of the ladders. | |||
* combine complementary strand to see if that displaces the ThT | |||
Revision as of 10:47, 16 April 2013
Gel Electrophoresis/Abs/thiol-DNA-ThT Rxn | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objectives
ProceduresPart one: Reconstituting DNA samples in water 1) Remove epi-test tubes from lyophilizer 2) Add 10 μL water to each sample and vortex to dissolve Part 2: Gel Electrophoresis. 1) Follow procedure outlined on 03/29/2013 2) Prepare the following 7 samples:
3) Load into gel 4) Stain with ehtidium bromide 5) Observe under UV Light Part 3: Preparing thiol-DNA/ThT solution
1) Dilute stock DNA (53.1μM) to 5.82μM 53.1μM DNA (x) = (500μL) (5.82μM) X = 54.8micL in 445.2 mic buffer 2) Mix 250μL thiol-DNA with 250μL 5μMThT 3) Heat at ~75°C for 25 minutes 4) Cool to room temperature DataObservationsThe absorbance data did not show a trend. The thiol-DNA/ThT/AuNP sample itself was the faintest shade of purple, which may be one reason we did not observe a distinguishable peak. Because of this, we decided to re-do the thiol-DNA/ThT reaction using a higher concentration of DNA (See Part 3 of the procedures). This allowed us to work with a higher concentration of gold nanoparticles since it's concentration is based off of the concentration of DNA used. Notes
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