User:Allison K. Alix/Notebook/Thesis Research/2013/04/16: Difference between revisions
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==Observations== | ==Observations== | ||
The absorbance data did not show a trend. The thiol-DNA/ThT/AuNP sample itself was the faintest shade of purple, which may be one reason we did not observe a distinguishable peak. Because of this, we decided to re-do the thiol-DNA/ThT reaction using a higher concentration of DNA (See Part 3 of the procedures). This allowed us to work with a higher concentration of gold nanoparticles since the DNA | The absorbance data did not show a trend. The thiol-DNA/ThT/AuNP sample itself was the faintest shade of purple, which may be one reason we did not observe a distinguishable peak. Because of this, we decided to re-do the thiol-DNA/ThT reaction using a higher concentration of DNA (See Part 3 of the procedures). This allowed us to work with a higher concentration of gold nanoparticles since it's concentration is based off of the concentration of DNA used. | ||
Revision as of 09:45, 16 April 2013
Gel Electrophoresis/Abs/thiol-DNA-ThT Rxn | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objectives
ProceduresPart one: Reconstituting DNA samples in water 1) Remove epi-test tubes from lyophilizer 2) Add 10 μL water to each sample and vortex to dissolve Part 2: Gel Electrophoresis. 1) Follow procedure outlined on 03/29/2013 2) Prepare the following 7 samples:
3) Load into gel 4) Stain with ehtidium bromide 5) Observe under UV Light Part 3: Preparing thiol-DNA/ThT solution
1) Dilute stock DNA (53.1μM) to 5.82μM 53.1μM DNA (x) = (500μL) (5.82μM) X = 54.8micL in 445.2 mic buffer 2) Mix 250μL thiol-DNA with 250μL 5μMThT 3) Heat at ~75°C for 25 minutes 4) Cool to room temperature DataObservationsThe absorbance data did not show a trend. The thiol-DNA/ThT/AuNP sample itself was the faintest shade of purple, which may be one reason we did not observe a distinguishable peak. Because of this, we decided to re-do the thiol-DNA/ThT reaction using a higher concentration of DNA (See Part 3 of the procedures). This allowed us to work with a higher concentration of gold nanoparticles since it's concentration is based off of the concentration of DNA used.
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