User:Allison K. Alix/Notebook/Thesis Research/2013/04/15: Difference between revisions
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Part 2: Reacting AuNP with DNA-thiol | Part 2: Reacting AuNP with DNA-thiol | ||
1. Mix 250μL 2.5μM ThT/727.5nM thiol-DNA synthesized [[User:Allison K. Alix/Notebook/Thesis Research/2013/04/05|04/05/2013]] with 250μL 4% TEA containing 100mM DTT | 1. Mix 250μL 2.5μM ThT/727.5nM thiol-DNA synthesized [[User:Allison K. Alix/Notebook/Thesis Research/2013/04/05|04/05/2013]] with 250μL 4% TEA containing 100mM DTT | ||
* New DNA concentration: 363.75nM, New ThT Concentration: 1.25μM | |||
2. React 10 minutes | 2. React 10 minutes | ||
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3. Extract DTT using 4 2mL aliquots of ethyl acetate. DTT partitions into ethyl acetate and DNA partitions into aqueous phase | 3. Extract DTT using 4 2mL aliquots of ethyl acetate. DTT partitions into ethyl acetate and DNA partitions into aqueous phase | ||
4. Mix 232.4μL ThT/thiol-DNA with 232.4μL AuNP and 35.2μL sodium citrate buffer | 4. Mix 232.4μL 1.25μM ThT/363.75nM thiol-DNA with 232.4μL 4.85nM AuNP and 35.2μL sodium citrate buffer | ||
* Original concentration AuNP 19.4nM, concentration of solution needs to be 1:75 AuNP:DNA | |||
363.75nM/74 = 4.85nM | |||
19.4nM (x) = 4.85nM (250μL) | |||
x = 62.5μL AuNP in 187.5μL flitered water | |||
5. React for 10 minutes | |||
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Revision as of 09:56, 15 April 2013
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Entry title
ProceduresPart 1: Repeating Gel Electrophoresis
Part 2: Reacting AuNP with DNA-thiol 1. Mix 250μL 2.5μM ThT/727.5nM thiol-DNA synthesized 04/05/2013 with 250μL 4% TEA containing 100mM DTT
2. React 10 minutes 3. Extract DTT using 4 2mL aliquots of ethyl acetate. DTT partitions into ethyl acetate and DNA partitions into aqueous phase 4. Mix 232.4μL 1.25μM ThT/363.75nM thiol-DNA with 232.4μL 4.85nM AuNP and 35.2μL sodium citrate buffer
363.75nM/74 = 4.85nM 19.4nM (x) = 4.85nM (250μL) x = 62.5μL AuNP in 187.5μL flitered water 5. React for 10 minutes |