User:Allison K. Alix/Notebook/Thesis Research/2013/04/15: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Objectives== | ||
* Repeat Gel Electrophoresis on same samples as 4/12/13 (Stained in ethidium bromide for too long | * Repeat Gel Electrophoresis on same samples as 4/12/13 (Stained in ethidium bromide for too long | ||
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5. React for 10 minutes | 5. React for 10 minutes | ||
6. Centrifuge 4 times, each time re-dispersing in HEPES buffer | 6. Centrifuge 4 times, each time re-dispersing in 500 μL 50mM HEPES buffer | ||
7. Save all supernatants and run Absorbance/Fluorescence Measurements on all | 7. Save all supernatants and run Absorbance/Fluorescence Measurements on all |
Latest revision as of 22:38, 26 September 2017
Project name | Main project page Previous entry Next entry |
Objectives
ProceduresPart 1: Repeating Gel Electrophoresis
Part 2: Reacting AuNP with DNA-thiol 1. Mix 250μL 2.5μM ThT/727.5nM thiol-DNA synthesized 04/05/2013 with 250μL 4% TEA containing 100mM DTT
2. React 10 minutes 3. Extract DTT using 4 2mL aliquots of ethyl acetate. DTT partitions into ethyl acetate and DNA partitions into aqueous phase 4. Mix 232.4μL 1.25μM ThT/363.75nM thiol-DNA with 232.4μL 4.85nM AuNP and 35.2μL sodium citrate buffer
363.75nM/74 = 4.85nM 19.4nM (x) = 4.85nM (250μL) x = 62.5μL AuNP in 187.5μL flitered water 5. React for 10 minutes 6. Centrifuge 4 times, each time re-dispersing in 500 μL 50mM HEPES buffer 7. Save all supernatants and run Absorbance/Fluorescence Measurements on all |