User:Allison K. Alix/Notebook/Thesis Research/2013/04/15: Difference between revisions

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Part 2: Reacting AuNP with DNA-thiol
Part 2: Reacting AuNP with DNA-thiol


1. Use 2.5μM ThT with 727.5nM DNA synthesized  
1. Mix 250μL 2.5μM ThT/727.5nM thiol-DNA synthesized [[User:Allison K. Alix/Notebook/Thesis Research/2013/04/05|04/05/2013]] with 250μL 4% TEA containing 100mM DTT
 
2. React 10 minutes
 
3. Extract DTT using 4 2mL aliquots of ethyl acetate. DTT partitions into ethyl acetate and DNA partitions into aqueous phase
 
4. Mix 232.4μL ThT/thiol-DNA with 232.4μL AuNP and 35.2μL sodium citrate buffer


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Revision as of 08:37, 15 April 2013

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Entry title

  • Repeat Gel Electrophoresis on same samples as 4/12/13 (Stained in ethidium bromide for too long
  • Bind AuNP to DNA-thiol hybrid

Procedures

Part 1: Repeating Gel Electrophoresis

  • Prepare samples the same way

Part 2: Reacting AuNP with DNA-thiol

1. Mix 250μL 2.5μM ThT/727.5nM thiol-DNA synthesized 04/05/2013 with 250μL 4% TEA containing 100mM DTT

2. React 10 minutes

3. Extract DTT using 4 2mL aliquots of ethyl acetate. DTT partitions into ethyl acetate and DNA partitions into aqueous phase

4. Mix 232.4μL ThT/thiol-DNA with 232.4μL AuNP and 35.2μL sodium citrate buffer


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