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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Gel Electrophoresis</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Gel Electrophoresis</span>
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==Procedures==
==Procedures==


'''Part 1: Gel Electrophoresis on DNA-AuNP hybrid'''
* Follow procedures outlined on [[User:Allison K. Alix/Notebook/Thesis Research/2013/03/29|03/29/2013]]
The following samples were observed
1) 4μL filtered water + 1μL 100bp DNA ladder + 1μL loading dye
2) 4μL filtered water + 1μL DNA/AuNP + 1μL loading dye
3) 4μL filtered water + 1μL supernatant 1 + 1μL loading dye
4) 4μL filtered water + 1μL supernatant 2 + 1μL loading dye
5) 4μL filtered water + 1μL supernatant 3 + 1μL loading dye
6) 4μL filtered water + 1μL supernatant 4 + 1μL loading dye
* Stain for ~10min in ethidium bromide


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Latest revision as of 22:37, 26 September 2017

Gel Electrophoresis Main project page
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Objectives

  • Complete Gel Electrophoresis on DNA-AuNP hybrid synthesized 3/18
  • Observe under UV light

Procedures

Part 1: Gel Electrophoresis on DNA-AuNP hybrid

The following samples were observed

1) 4μL filtered water + 1μL 100bp DNA ladder + 1μL loading dye

2) 4μL filtered water + 1μL DNA/AuNP + 1μL loading dye

3) 4μL filtered water + 1μL supernatant 1 + 1μL loading dye

4) 4μL filtered water + 1μL supernatant 2 + 1μL loading dye

5) 4μL filtered water + 1μL supernatant 3 + 1μL loading dye

6) 4μL filtered water + 1μL supernatant 4 + 1μL loading dye

  • Stain for ~10min in ethidium bromide


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