User:Allison K. Alix/Notebook/Thesis Research/2013/04/11

From OpenWetWare

< User:Allison K. Alix | Notebook | Thesis Research | 2013 | 04(Difference between revisions)
Jump to: navigation, search
(Objectives)
Current revision (11:25, 12 April 2013) (view source)
(Procedures)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
+
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Gel Electrophoresis</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
|-
Line 14: Line 14:
==Procedures==
==Procedures==
 +
'''Part 1: Gel Electrophoresis on DNA-AuNP hybrid'''
 +
 +
* Follow procedures outlined on [[User:Allison K. Alix/Notebook/Thesis Research/2013/03/29|03/29/2013]]
 +
 +
The following samples were observed
 +
 +
1) 4μL filtered water + 1μL 100bp DNA ladder + 1μL loading dye
 +
 +
2) 4μL filtered water + 1μL DNA/AuNP + 1μL loading dye
 +
 +
3) 4μL filtered water + 1μL supernatant 1 + 1μL loading dye
 +
 +
4) 4μL filtered water + 1μL supernatant 2 + 1μL loading dye
 +
 +
5) 4μL filtered water + 1μL supernatant 3 + 1μL loading dye
 +
 +
6) 4μL filtered water + 1μL supernatant 4 + 1μL loading dye
 +
 +
* Stain for ~10min in ethidium bromide
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Current revision

Gel Electrophoresis Main project page
Previous entry      Next entry

Objectives

  • Complete Gel Electrophoresis on DNA-AuNP hybrid synthesized 3/18
  • Observe under UV light

Procedures

Part 1: Gel Electrophoresis on DNA-AuNP hybrid

The following samples were observed

1) 4μL filtered water + 1μL 100bp DNA ladder + 1μL loading dye

2) 4μL filtered water + 1μL DNA/AuNP + 1μL loading dye

3) 4μL filtered water + 1μL supernatant 1 + 1μL loading dye

4) 4μL filtered water + 1μL supernatant 2 + 1μL loading dye

5) 4μL filtered water + 1μL supernatant 3 + 1μL loading dye

6) 4μL filtered water + 1μL supernatant 4 + 1μL loading dye

  • Stain for ~10min in ethidium bromide


Personal tools