User:Allison K. Alix/Notebook/Thesis Research/2013/04/11: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Gel Electrophoresis</span> | ||
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== | ==Objectives== | ||
* Complete Gel Electrophoresis on DNA-AuNP hybrid synthesized 3/18 | |||
* Observe under UV light | |||
==Procedures== | |||
'''Part 1: Gel Electrophoresis on DNA-AuNP hybrid''' | |||
* Follow procedures outlined on [[User:Allison K. Alix/Notebook/Thesis Research/2013/03/29|03/29/2013]] | |||
The following samples were observed | |||
1) 4μL filtered water + 1μL 100bp DNA ladder + 1μL loading dye | |||
2) 4μL filtered water + 1μL DNA/AuNP + 1μL loading dye | |||
3) 4μL filtered water + 1μL supernatant 1 + 1μL loading dye | |||
4) 4μL filtered water + 1μL supernatant 2 + 1μL loading dye | |||
5) 4μL filtered water + 1μL supernatant 3 + 1μL loading dye | |||
6) 4μL filtered water + 1μL supernatant 4 + 1μL loading dye | |||
* Stain for ~10min in ethidium bromide | |||
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Revision as of 08:25, 12 April 2013
 Gel Electrophoresis
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Objectives
ProceduresPart 1: Gel Electrophoresis on DNA-AuNP hybrid
The following samples were observed 1) 4μL filtered water + 1μL 100bp DNA ladder + 1μL loading dye 2) 4μL filtered water + 1μL DNA/AuNP + 1μL loading dye 3) 4μL filtered water + 1μL supernatant 1 + 1μL loading dye 4) 4μL filtered water + 1μL supernatant 2 + 1μL loading dye 5) 4μL filtered water + 1μL supernatant 3 + 1μL loading dye 6) 4μL filtered water + 1μL supernatant 4 + 1μL loading dye
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