User:Allison K. Alix/Notebook/Thesis Research/2013/04/09

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(Objectives)
Current revision (11:19, 12 April 2013) (view source)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Reacting Ethane thiol with AuNP</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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* React ethane-thiol with AuNP
* React ethane-thiol with AuNP
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==Procedures==
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'''Part 1: Preparation of 1mL of 2.98μM ethane thiol'''
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WARNING: ethanethiol is very volatile. Handle under the hood only wearing PPE.
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* original concentration of AuNP = 19.4nM
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* desired concentration of ethanethiol = 19.4nM x(15393) = 298624.2nM = 298μM
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* density of ethanethiol = 0.839g/mL
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1) Find the concentration of 0.0125mL pure ethanethiol in 5mL water
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0.839g/mL x 0.0125mL = 0.0104875g
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0.0104875g/(62.13 g/mol) = 1.68799 x 10<sup>-4</sup> mol
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1.68799 x 10<sup>-4</sup> mol/5mL x (1000mL/1L) = 0.034M = 34mM
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340mM (x) = (30mM)(1mL)
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x = 0.088mL = 88μL in 912μL H<sub>2</sub>O
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30mM (x) = 3mM (1mL)
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x = 100μL in 900μL
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3mM(x) = 298μM (1mL)
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x = 99μL in 901μL to obtain 298μM ethanethiol
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2) Reacting AuNP with ethanethiol
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* react 200μL 298μM ethane thiol with 200μL 19.4nM AuNP (synthesized by Dr. Miller)
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'''Part 2: Measure absorbance/fluorescence of AuNP-MB'''
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'''Part 3: Measure absorbance/fluorescence of AuNP-ThT'''
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==Data==
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Part 2:
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[[Image:ThiolDNA_MB_pic_UV-Vis.png‎]]
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UV-Vis Spectrum of thiol-DNA/MB hybrid
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[[Image:ThiolDNA_MB_pic.png]]
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Fluorescence Spectrum of thiol-DNA/MB hybrid
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Part 3:
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[[Image:ThiolDNA_ThT_pic_UV-Vis.png‎]]
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UV-Vis Spectrum of thiol-DNA/ThT hybrid
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[[Image:ThiolDNA ThT pic.png]]
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Fluorescence Spectrum of thiol-DNA/ThT hybrid

Current revision

Reacting Ethane thiol with AuNP Main project page
Previous entry      Next entry

Objectives

  • Take Abs and Fluorescence measurements of DNA-MB and DNA-ThT prepared on 4/4/13
  • React ethane-thiol with AuNP

Procedures

Part 1: Preparation of 1mL of 2.98μM ethane thiol

WARNING: ethanethiol is very volatile. Handle under the hood only wearing PPE.

  • original concentration of AuNP = 19.4nM
  • desired concentration of ethanethiol = 19.4nM x(15393) = 298624.2nM = 298μM
  • density of ethanethiol = 0.839g/mL

1) Find the concentration of 0.0125mL pure ethanethiol in 5mL water

0.839g/mL x 0.0125mL = 0.0104875g

0.0104875g/(62.13 g/mol) = 1.68799 x 10-4 mol

1.68799 x 10-4 mol/5mL x (1000mL/1L) = 0.034M = 34mM


340mM (x) = (30mM)(1mL)

x = 0.088mL = 88μL in 912μL H2O

30mM (x) = 3mM (1mL)

x = 100μL in 900μL

3mM(x) = 298μM (1mL)

x = 99μL in 901μL to obtain 298μM ethanethiol

2) Reacting AuNP with ethanethiol

  • react 200μL 298μM ethane thiol with 200μL 19.4nM AuNP (synthesized by Dr. Miller)

Part 2: Measure absorbance/fluorescence of AuNP-MB

Part 3: Measure absorbance/fluorescence of AuNP-ThT

Data

Part 2:

Image:ThiolDNA_MB_pic_UV-Vis.png‎

UV-Vis Spectrum of thiol-DNA/MB hybrid

Image:ThiolDNA_MB_pic.png

Fluorescence Spectrum of thiol-DNA/MB hybrid

Part 3:

Image:ThiolDNA_ThT_pic_UV-Vis.png‎

UV-Vis Spectrum of thiol-DNA/ThT hybrid

Image:ThiolDNA ThT pic.png

Fluorescence Spectrum of thiol-DNA/ThT hybrid



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