User:Allison K. Alix/Notebook/Thesis Research/2013/04/05

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(Procedures)
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==Entry title==
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==Objectives==
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* Insert content here...
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* Mix DNA with higher concentration of ThT
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* Measure absorbance and fluorescence of DNA/ThT hybrid
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==Procedures==
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Part 1: Mixing thiol-DNA with ThT
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1) Mix 200μL of 5μM ThT with 200μL 1.455μM thiol-DNA
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* Note that new concentrations of reactants in solution are 2.5μM ThT and 0.7275μM DNA
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2) Place on heat block at ~76°C for 25 minutes
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3) Allow solution to cool on heat block until at room temperature
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4) Take absorbance and fluorescence measurements
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* AuNP will then be added to this solution
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Part 2: Mixing thiol-DNA with MB
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Prepare MB (need at least 100μL)
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12μL (7.31μM) = x(100μL)
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x = 0.8772μM = 877.2nM
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mix 100μL with 100μL 727.5nM DNA

Revision as of 10:38, 5 April 2013

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Objectives

  • Mix DNA with higher concentration of ThT
  • Measure absorbance and fluorescence of DNA/ThT hybrid

Procedures

Part 1: Mixing thiol-DNA with ThT

1) Mix 200μL of 5μM ThT with 200μL 1.455μM thiol-DNA

  • Note that new concentrations of reactants in solution are 2.5μM ThT and 0.7275μM DNA

2) Place on heat block at ~76°C for 25 minutes

3) Allow solution to cool on heat block until at room temperature

4) Take absorbance and fluorescence measurements

  • AuNP will then be added to this solution

Part 2: Mixing thiol-DNA with MB

Prepare MB (need at least 100μL)

12μL (7.31μM) = x(100μL)

x = 0.8772μM = 877.2nM

mix 100μL with 100μL 727.5nM DNA



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