User:Allison K. Alix/Notebook/Thesis Research/2013/04/04: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Abs and Fluor Measurements</span> | ||
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==Observations== | ==Observations== | ||
* The concentration of ThT used in this solution was likely too low. Although ThT was in excess, the absorbance seen on the UV-Vis spectrum was very low. It is interesting to note that the DNA-ThT hybrid did show slight fluorescence intensity. Because there is a possibility that this signal was a background signal coming from the ThT, a fluorescence spectrum of that same concentration of ThT alone in solution was also taken (See Data below) | |||
==Data== | |||
Absorbance: | |||
[[Image:ThiolDNAThThybridUVVisspec.png]] | |||
Fluorescence: | |||
[[Image:ThiolDNAThThybridFluorescencespec.png]] | |||
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Revision as of 08:16, 12 April 2013
Abs and Fluor Measurements | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objectives
ProceduresPart 1: Measure absorbance values of hybrid thiol-DNA/ThT fluorescence: 1) excitation at 400. Start 410, end 610 (according to absorbance spec 2) excitation at 440 start at 450 end 650 3) excitation at 417 start at 427 end 650 Part 2: Measure fluorescence of ThT and DNA alone Observations
DataAbsorbance: Fluorescence: |