User:Allison K. Alix/Notebook/Thesis Research/2013/04/04: Difference between revisions
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==Observations== | ==Observations== | ||
* The concentration of ThT used in this solution was likely too low. Although ThT was in excess, the absorbance seen on the UV-Vis spectrum was very low. It is interesting to note that the DNA-ThT hybrid did show slight fluorescence intensity. Because there is a possibility that this signal was a background signal coming from the ThT, a fluorescence spectrum of that same concentration of ThT alone in solution was also taken. | |||
Revision as of 07:05, 5 April 2013
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Objectives
ProceduresPart 1: Measure absorbance values of hybrid thiol-DNA/ThT fluorescence: 1) excitation at 400. Start 410, end 610 (according to absorbance spec 2) excitation at 440 start at 450 end 650 3) excitation at 417 start at 427 end 650 Part 2: Measure fluorescence of ThT and DNA alone Observations
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