User:Allison K. Alix/Notebook/Thesis Research/2013/04/02: Difference between revisions

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==Procedures==
==Procedures==


Part 1: Reacting DNA with MB first, followed by a reaction of the DNA-MB hybrid with AuNP
'''Part 1: Reacting DNA with MB first, followed by a reaction of the DNA-MB hybrid with AuNP'''


1) Prepare 727.5nm 8-repeat DNA-thiol and 800nm MB
1) Prepare 727.5nm 8-repeat DNA-thiol and 800nm MB

Revision as of 09:05, 2 April 2013

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Objectives

  • Bind DNA to MB and AuNP
  • React AuNP with ethanethiol

Procedures

Part 1: Reacting DNA with MB first, followed by a reaction of the DNA-MB hybrid with AuNP

1) Prepare 727.5nm 8-repeat DNA-thiol and 800nm MB

  • 727.5nm 8-repeat DNA-thiol

(Original concentration of DNA is 53.1μM)

53.1μM(x)=(500μL)(1455nm)

x= 13.7μL in 486.3μL H2O

1455nM(x) = 727.5nm(200μL)

x= 100μL 1455nM in 100μL H2O

2) Prepare 800nm MB

(Original concentration of MB is 7.31μM)

7130nm(x) = (800nM)(50μL)

x = 5.47μL 7.31μM in 44.53 μL H2O

3) Mix 50μL 727.5nM DNA with 50μL 800nM MB

4) Hybridize by placing mixture on heat block @ 76°C for ~25minutes

5) Turn off heat block and allow cooling to room temperature






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