User:Allison K. Alix/Notebook/Thesis Research/2013/04/02: Difference between revisions
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1) Prepare 727.5nm 8-repeat DNA-thiol and 800nm MB | 1) Prepare 727.5nm 8-repeat DNA-thiol and 800nm MB | ||
a) 727.5nm 8-repeat DNA-thiol | |||
(Original concentration of DNA is 53.1μM) | (Original concentration of DNA is 53.1μM) | ||
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x= 100μL 1455nM in 100μL H<sub>2</sub>O | x= 100μL 1455nM in 100μL H<sub>2</sub>O | ||
b) Prepare 800nm MB | |||
(Original concentration of MB is 7.31μM) | (Original concentration of MB is 7.31μM) | ||
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5) Turn off heat block and allow cooling to room temperature | 5) Turn off heat block and allow cooling to room temperature | ||
'''Part 2: Reacting DNA with ThT first, followed by a reaction of the DNA-ThT hybrid with AuNP''' | |||
1) Prepare 727.5nM DNA (see Part 1, step 1a) | |||
2) Prepare 800nM ThT | |||
*Note that the ThT prepared on [[Link title]] | |||
Revision as of 09:08, 2 April 2013
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Objectives
ProceduresPart 1: Reacting DNA with MB first, followed by a reaction of the DNA-MB hybrid with AuNP 1) Prepare 727.5nm 8-repeat DNA-thiol and 800nm MB a) 727.5nm 8-repeat DNA-thiol (Original concentration of DNA is 53.1μM) 53.1μM(x)=(500μL)(1455nm) x= 13.7μL in 486.3μL H2O 1455nM(x) = 727.5nm(200μL) x= 100μL 1455nM in 100μL H2O b) Prepare 800nm MB (Original concentration of MB is 7.31μM) 7130nm(x) = (800nM)(50μL) x = 5.47μL 7.31μM in 44.53 μL H2O 3) Mix 50μL 727.5nM DNA with 50μL 800nM MB 4) Hybridize by placing mixture on heat block @ 76°C for ~25minutes 5) Turn off heat block and allow cooling to room temperature Part 2: Reacting DNA with ThT first, followed by a reaction of the DNA-ThT hybrid with AuNP 1) Prepare 727.5nM DNA (see Part 1, step 1a) 2) Prepare 800nM ThT
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