User:Allison K. Alix/Notebook/Thesis Research/2013/02/12

From OpenWetWare

< User:Allison K. Alix | Notebook | Thesis Research | 2013 | 02
Revision as of 13:15, 19 February 2013 by Allison K. Alix (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
Fluorescence Measurements Main project page
Previous entry      Next entry

Objectives

  • Take Fluorescence Measurements of samples from 2/5/2013
  • We are expecting to see a decrease in signal with decreasing DNA concentration

Spectra

Image:4repeatDNAThTfluorgraph.jpg

Image:8repeatDNAThTfluorgraph.jpg

Image:Fluorintcomp1.5micM.png‎

Image:Fluorintcomp2.0micM.png‎

Image:Fluorintcomp2.5micM.png‎

Image:Fluorintcomp3.0micM.png‎

Image:Fluorintcomp3.5micM.png‎

Observations

4 repeat telomeric DNA samples:

There was only a trend of increasing intensity with increasing concentration of DNA seen in the 1.5μM, 3.0μM, and 3.5μM samples. The 2.0μM and 2.5μM samples were very much out of order. Next step is to plot intensity at max peak vs concentration.


Image:Fluorintvsconc4strandpic.png

Image:Fluorintvsconc8strandpic.png


Personal tools