User:Allison K. Alix/Notebook/Thesis Research/2013/02/12

From OpenWetWare

< User:Allison K. Alix | Notebook | Thesis Research | 2013 | 02(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/02/12 Entry for User:Allison_K._Alix/Notebook/Thesis_Research)
Current revision (13:15, 19 February 2013) (view source)
(Spectra)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
+
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Fluorescence Measurements</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==Objectives==
-
* Insert content here...
+
 +
*Take Fluorescence Measurements of samples from [[User:Allison_K._Alix/Notebook/Thesis_Research/2013/02/05|2/5/2013]]
 +
* We are expecting to see a decrease in signal with decreasing DNA concentration
 +
 +
=Spectra=
 +
 +
[[Image:4repeatDNAThTfluorgraph.jpg]]
 +
 +
[[Image:8repeatDNAThTfluorgraph.jpg]]
 +
 +
[[Image:Fluorintcomp1.5micM.png‎]]
 +
 +
[[Image:Fluorintcomp2.0micM.png‎]]
 +
 +
[[Image:Fluorintcomp2.5micM.png‎]]
 +
 +
[[Image:Fluorintcomp3.0micM.png‎]]
 +
 +
[[Image:Fluorintcomp3.5micM.png‎]]
 +
 +
=Observations=
 +
 +
4 repeat telomeric DNA samples:
 +
 +
There was only a trend of increasing intensity with increasing concentration of DNA seen in the 1.5μM, 3.0μM, and 3.5μM samples. The 2.0μM and 2.5μM samples were very much out of order. Next step is to plot intensity at max peak vs concentration.
 +
 +
 +
[[Image:Fluorintvsconc4strandpic.png]]
 +
 +
[[Image:Fluorintvsconc8strandpic.png]]
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Current revision

Fluorescence Measurements Main project page
Previous entry      Next entry

Objectives

  • Take Fluorescence Measurements of samples from 2/5/2013
  • We are expecting to see a decrease in signal with decreasing DNA concentration

Spectra

Image:4repeatDNAThTfluorgraph.jpg

Image:8repeatDNAThTfluorgraph.jpg

Image:Fluorintcomp1.5micM.png‎

Image:Fluorintcomp2.0micM.png‎

Image:Fluorintcomp2.5micM.png‎

Image:Fluorintcomp3.0micM.png‎

Image:Fluorintcomp3.5micM.png‎

Observations

4 repeat telomeric DNA samples:

There was only a trend of increasing intensity with increasing concentration of DNA seen in the 1.5μM, 3.0μM, and 3.5μM samples. The 2.0μM and 2.5μM samples were very much out of order. Next step is to plot intensity at max peak vs concentration.


Image:Fluorintvsconc4strandpic.png

Image:Fluorintvsconc8strandpic.png


Personal tools