User:Allison K. Alix/Notebook/Thesis Research/2013/02/12

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(Observations)
Current revision (14:15, 19 February 2013) (view source)
(Spectra)
 
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[[Image:8repeatDNAThTfluorgraph.jpg]]
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=Observations=
=Observations=
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There was only a trend of increasing intensity with increasing concentration of DNA seen in the 1.5μM, 3.0μM, and 3.5μM samples. The 2.0μM and 2.5μM samples were very much out of order. Next step is to plot intensity at max peak vs concentration.
There was only a trend of increasing intensity with increasing concentration of DNA seen in the 1.5μM, 3.0μM, and 3.5μM samples. The 2.0μM and 2.5μM samples were very much out of order. Next step is to plot intensity at max peak vs concentration.
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[[Image:Fluorintvsconc4strandpic.png]]
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[[Image:Fluorintvsconc8strandpic.png]]
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Current revision

Fluorescence Measurements Main project page
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Objectives

  • Take Fluorescence Measurements of samples from 2/5/2013
  • We are expecting to see a decrease in signal with decreasing DNA concentration

Spectra

Image:4repeatDNAThTfluorgraph.jpg

Image:8repeatDNAThTfluorgraph.jpg

Image:Fluorintcomp1.5micM.png‎

Image:Fluorintcomp2.0micM.png‎

Image:Fluorintcomp2.5micM.png‎

Image:Fluorintcomp3.0micM.png‎

Image:Fluorintcomp3.5micM.png‎

Observations

4 repeat telomeric DNA samples:

There was only a trend of increasing intensity with increasing concentration of DNA seen in the 1.5μM, 3.0μM, and 3.5μM samples. The 2.0μM and 2.5μM samples were very much out of order. Next step is to plot intensity at max peak vs concentration.


Image:Fluorintvsconc4strandpic.png

Image:Fluorintvsconc8strandpic.png


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