User:Allison K. Alix/Notebook/Thesis Research/2013/02/05

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Current revision (11:29, 12 February 2013) (view source)
(Absorbance Spectra)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Absorbance Measurements</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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-Verify that there is no absorbance change with increasing concentration of DNA  
-Verify that there is no absorbance change with increasing concentration of DNA  
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==List of Samples==
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1) Moelcular Beacon [6.49 nM]
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2) 3.5μM ThT
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3) 5.0μM ZnPPIX
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4) 3.5μM ThT/5.0μM ZnPPIX hybrid
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5) 1μM 4-repeat-telomeric DNA
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6) 1μM 8-repeat-telomeric DNA
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7) 1μM 4-repeat/5μM ZnPPIX hybrid
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8) 1μM 8-repeat/5μM ZnPPIX hybrid
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9) 3.5μM ThT/3.5μM 4-repeat
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10) 3.5μM ThT/3.0μM 4-repeat
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11) 3.5μM ThT/2.5μM 4-repeat
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12) 3.5μM ThT/2.0μM 4-repeat
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13) 3.5μM ThT/1.5μM 4-repeat
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14) 3.5μM ThT/3.5μM 8-repeat
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15) 3.5μM ThT/3.0μM 8-repeat
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16) 3.5μM ThT/2.5μM 8-repeat
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17) 3.5μM ThT/2.0μM 8-repeat
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18) 3.5μM ThT/1.5μM 8-repeat
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=Absorbance Spectra=
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[[Image:4repeatDNAThTgraph.jpg]]
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[[Image:8repeatDNAThTgraph.jpg]]
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Current revision

Absorbance Measurements Main project page
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Objectives

-Take absorbance spectra of all samples prepared on 1/29/13

-Verify that there is no absorbance change with increasing concentration of DNA

List of Samples

1) Moelcular Beacon [6.49 nM]

2) 3.5μM ThT

3) 5.0μM ZnPPIX

4) 3.5μM ThT/5.0μM ZnPPIX hybrid

5) 1μM 4-repeat-telomeric DNA

6) 1μM 8-repeat-telomeric DNA

7) 1μM 4-repeat/5μM ZnPPIX hybrid

8) 1μM 8-repeat/5μM ZnPPIX hybrid

9) 3.5μM ThT/3.5μM 4-repeat

10) 3.5μM ThT/3.0μM 4-repeat

11) 3.5μM ThT/2.5μM 4-repeat

12) 3.5μM ThT/2.0μM 4-repeat

13) 3.5μM ThT/1.5μM 4-repeat

14) 3.5μM ThT/3.5μM 8-repeat

15) 3.5μM ThT/3.0μM 8-repeat

16) 3.5μM ThT/2.5μM 8-repeat

17) 3.5μM ThT/2.0μM 8-repeat

18) 3.5μM ThT/1.5μM 8-repeat

Absorbance Spectra

Image:4repeatDNAThTgraph.jpg


Image:8repeatDNAThTgraph.jpg



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