User:Allison K. Alix/Notebook/Thesis Research/2013/02/05: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Absorbance Measurements</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Objectives== | ||
-Take absorbance spectra of all samples prepared on 1/29/13 | |||
-Verify that there is no absorbance change with increasing concentration of DNA | |||
==List of Samples== | |||
1) Moelcular Beacon [6.49 nM] | |||
2) 3.5μM ThT | |||
3) 5.0μM ZnPPIX | |||
4) 3.5μM ThT/5.0μM ZnPPIX hybrid | |||
5) 1μM 4-repeat-telomeric DNA | |||
6) 1μM 8-repeat-telomeric DNA | |||
7) 1μM 4-repeat/5μM ZnPPIX hybrid | |||
8) 1μM 8-repeat/5μM ZnPPIX hybrid | |||
9) 3.5μM ThT/3.5μM 4-repeat | |||
10) 3.5μM ThT/3.0μM 4-repeat | |||
11) 3.5μM ThT/2.5μM 4-repeat | |||
12) 3.5μM ThT/2.0μM 4-repeat | |||
13) 3.5μM ThT/1.5μM 4-repeat | |||
14) 3.5μM ThT/3.5μM 8-repeat | |||
15) 3.5μM ThT/3.0μM 8-repeat | |||
16) 3.5μM ThT/2.5μM 8-repeat | |||
17) 3.5μM ThT/2.0μM 8-repeat | |||
18) 3.5μM ThT/1.5μM 8-repeat | |||
=Absorbance Spectra= | |||
[[Image:4repeatDNAThTgraph.jpg]] | |||
[[Image:8repeatDNAThTgraph.jpg]] | |||
Latest revision as of 22:25, 26 September 2017
 Absorbance Measurements
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Objectives-Take absorbance spectra of all samples prepared on 1/29/13 -Verify that there is no absorbance change with increasing concentration of DNA List of Samples1) Moelcular Beacon [6.49 nM] 2) 3.5μM ThT 3) 5.0μM ZnPPIX 4) 3.5μM ThT/5.0μM ZnPPIX hybrid 5) 1μM 4-repeat-telomeric DNA 6) 1μM 8-repeat-telomeric DNA 7) 1μM 4-repeat/5μM ZnPPIX hybrid 8) 1μM 8-repeat/5μM ZnPPIX hybrid 9) 3.5μM ThT/3.5μM 4-repeat 10) 3.5μM ThT/3.0μM 4-repeat 11) 3.5μM ThT/2.5μM 4-repeat 12) 3.5μM ThT/2.0μM 4-repeat 13) 3.5μM ThT/1.5μM 4-repeat 14) 3.5μM ThT/3.5μM 8-repeat 15) 3.5μM ThT/3.0μM 8-repeat 16) 3.5μM ThT/2.5μM 8-repeat 17) 3.5μM ThT/2.0μM 8-repeat 18) 3.5μM ThT/1.5μM 8-repeat Absorbance Spectra
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