User:Allison K. Alix/Notebook/Thesis Research/2013/01/29

(Difference between revisions)

Revision as of 20:12, 4 February 2013

Project name

-Repeat ZnPPIX and DNA hybridizations in 5:1 ratios instead of 1:1

-Hybridize ThT and DNA strands

ZnPPIX:

5μM ZnPPIX already prepared

1. Prepare 1 μM 4 telomeric repeat DNA:

265.6μM (x) = 1μM (100μL)

x = 2.656 μL in 97.344 μL H₂O

2. Prepare 1 μM 8 telomeric repeat DNA

142.8μM (x) = 1μM (100μL)

x = 1.428 μL in 98.572 μL H₂O

ThT

1. prepare 5mM tris-HCl buffer solution

121.4 g/mol x 5mmol/L x 1mol/1000mmol x 50 mL x 1L/1000mL = 0.030285 g tris in 50 mL H₂O

- Add 1 N HCl (~8 drops) to obtain pH ~ 7.25

2. prepare 3.5 μM ThT

318.86 g/mol x 3.5mmol/L x 1mol/1000mmol x 10mL x 1L/1000mL = 0.0111601 g in 10mL H₂O -> 3.5mM

(3.5 x 10^-3 M) (x) = (3.5 x 10^-6 M) (10mL)

x = 0.01mL in 9.99mL H₂O

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 . prepare 5mM tris-HCl buffer solution
-.4 g/mol x 5mmol/L x 1mol/1000mmol x 50 mL x 1L/1000mL = 0.030285 g in 50 mL
+.4 g/mol x 5mmol/L x 1mol/1000mmol x 50 mL x 1L/1000mL = 0.030285 g tris in 50 mL H<sub>2</sub>O
 - Add 1 N HCl (~8 drops) to obtain pH ~ 7.25
-.
+. prepare 3.5 μM ThT
+.86 g/mol x 3.5mmol/L x 1mol/1000mmol x 10mL x 1L/1000mL = 0.0111601 g in 10mL H<sub>2</sub>O -> 3.5mM
+(3.5 x 10^-3 M) (x) = (3.5 x 10^-6 M) (10mL)
+x = 0.01mL in 9.99mL H<sub>2</sub>O