User:Allison K. Alix/Notebook/Thesis Research/2013/01/29: Difference between revisions
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1. prepare 5mM tris-HCl buffer solution | 1. prepare 5mM tris-HCl buffer solution | ||
121.4 g/mol x 5mmol/L x 1mol/1000mmol x 50 mL x 1L/1000mL = 0.030285 g in 50 mL | |||
- Add 1 N HCl (~8 drops) to obtain pH ~ 7.25 | |||
2. | 2. | ||
Revision as of 17:08, 4 February 2013
 Project name
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Objectives-Repeat ZnPPIX and DNA hybridizations in 5:1 ratios instead of 1:1 -Hybridize ThT and DNA strands ProceduresZnPPIX: 5μM ZnPPIX already prepared 1. Prepare 1 μM 4 telomeric repeat DNA: 265.6μM (x) = 1μM (100μL) x = 2.656 μL in 97.344 μL H2O 2. Prepare 1 μM 8 telomeric repeat DNA 142.8μM (x) = 1μM (100μL) x = 1.428 μL in 98.572 μL H2O ThT 1. prepare 5mM tris-HCl buffer solution 121.4 g/mol x 5mmol/L x 1mol/1000mmol x 50 mL x 1L/1000mL = 0.030285 g in 50 mL - Add 1 N HCl (~8 drops) to obtain pH ~ 7.25 2.
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