User:Allison K. Alix/Notebook/Thesis Research/2013/01/29: Difference between revisions
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3. Prepare 3.5μM, 3μM, 2.5μM, 2.0μM, and 1.5μM solutions of 4 telomeric repeat DNA | 3. Prepare 3.5μM, 3μM, 2.5μM, 2.0μM, and 1.5μM solutions of 4 telomeric repeat DNA | ||
a) (265.6μM)(x) = (3.5μM) (250μL) | a) (265.6μM)(x) = (3.5μM) (250μL) | ||
::x= '''3.29μL''' in 246.71μL tris-HCl buffer | ::x= '''3.29μL''' in 246.71μL tris-HCl buffer | ||
b) (265.6μM)(x) = (3.0μM) (250μL) | b) (265.6μM)(x) = (3.0μM) (250μL) | ||
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::x= '''2.35μL''' in 247.65μL tris-HCl buffer | ::x= '''2.35μL''' in 247.65μL tris-HCl buffer | ||
d) (265.6μM)(x) = (2.0μM) (250μL) | d) (265.6μM)(x) = (2.0μM) (250μL) | ||
::x= 1.88μL in 248.12μL tris-HCl buffer | ::x= 1.88μL in 248.12μL tris-HCl buffer | ||
e) (265.6μM)(x) = (1.5μM) (250μL) | e) (265.6μM)(x) = (1.5μM) (250μL) |
Revision as of 17:21, 4 February 2013
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Objectives-Repeat ZnPPIX and DNA hybridizations in 5:1 ratios instead of 1:1 -Hybridize ThT and DNA strands ProceduresZnPPIX: 5μM ZnPPIX already prepared 1. Prepare 1 μM 4 telomeric repeat DNA: 265.6μM (x) = 1μM (100μL) x = 2.656 μL in 97.344 μL H2O 2. Prepare 1 μM 8 telomeric repeat DNA 142.8μM (x) = 1μM (100μL) x = 1.428 μL in 98.572 μL H2O ThT: 1. prepare 5mM tris-HCl buffer solution 121.4 g/mol x 5mmol/L x 1mol/1000mmol x 50 mL x 1L/1000mL = 0.030285 g tris in 50 mL H2O - Add 1 N HCl (~8 drops) to obtain pH ~ 7.25 2. prepare 3.5 μM ThT 318.86 g/mol x 3.5mmol/L x 1mol/1000mmol x 10mL x 1L/1000mL = 0.0111601 g in 10mL H2O -> 3.5mM (3.5 x 10^-3 M) (x) = (3.5 x 10^-6 M) (10mL) x = 0.01mL in 9.99mL H2O 3. Prepare 3.5μM, 3μM, 2.5μM, 2.0μM, and 1.5μM solutions of 4 telomeric repeat DNA
c) (265.6μM)(x) = (2.5μM) (250μL)
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